|
KMID : 0379120200480040389
|
|
Korean Journal of Mycology
2020 Volume.48 No. 4 p.389 ~ p.396
|
|
|
Development and Application of a Cleaved Amplified Polymorphic Sequence Marker for Discriminating A Mating Type Alleles of Lentinula edodes
|
|
Park Mi-Jeong
Ryoo Rhim
Jang Yeong-Seon
Ka Kang-Hyeon
|
|
|
Abstract
|
|
|
|
Lentinula edodes is one of the most widely consumed edible mushrooms in Korea. Mating in L. edodes is regulated by a tetrapolar system, and two unlinked genetic loci, A and B, are known to be major determinants of the mating types, as reported in other heterothallic basidiomycetes. The A locus of L. edodes encodes a pair of homeodomain (HD) transcription factors. The highly variable N-termini of these HD transcription factors contribute to the diversity among the A mating types. In this study, we developed a cleaved amplified polymorphic sequence (CAPS) marker to discriminate 11 different A mating type alleles predominant among both cultivated and wild strains. Amplification of the variable region of the A locus followed by digestion with HaeIII and EcoRI restriction enzymes enabled successful discrimination among the 11 A mating type alleles. We also evaluated the applicability of this method in the identification of two A mating types of a dikaryotic strain
|
|
|
KEYWORD
|
|
|
Cleaved amplified polymorphic sequence (CAPS) marker, Lentinula edodes, Mating type, Mushroom breeding, Restriction enzyme
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|